Effect of Probing Depth on Fluorescence Signal During Cardiac Optical Mapping
Xu Zhenghong1£¬2£¬Jin Yinbin1£¬2£¬Zhang Zhenxi1£¬Wang
Jing1
£¨1£®Key Laboratory of Biomedical Information Engineering of Education Ministry£¬Xi'an¡¡Jiaotong¡¡University£¬Xi'an
710049£¬China£»2£®School of Electrical Engineering£¬Xi'an¡¡Jiaotong¡¡University£¬Xi'an
710049£¬China£©
Abstract£ºTo investigate the effect of different probing depths during cardiac optical
mapping£¬ the electrical transmural propagation model, and the fluorescence transport
model were adopted to simulate the distribution of transmembrane potentials during the
transmural propagation and the fluorescence signals coming form three different optical
probing depths£® The depthª²weighted optical action potential is compared with the
action potential of the top layer, and the discrepancy is analyzed£® The results indicate
that the effect of optical probing depth may be ignored as most of the fluorescence only
comes from the subsurface £¨less than 0.5 mm£© but the discrepancy of the action
upstroke duration£¬ the signal amplitude and duration between two action potentials are
enhanced with the increase of the probing depth greater than or equal to 0.5 mm£©£¬
especially in the spiral wave activity£® So it is necessary to correspondingly correct
the judgments of the physiological states of myocardial cells generally obtained by
electrodes from the tissue surface£®
Keywords£ºprobing depth£» fluorescence£» fluorescence transport model£» cardiac
optical mapping