Journal of Xi'an Jiaotong University

Effect of Pulsed Current Magnetic Fields on Neural Stem Cell Proliferation in Hippocampus of Stressed Rat
Li Hui¹,Zhu Zhongliang²,Li Xia³,Jia Ning²,Song Liang²,Chen Rui¹,Yao Xueling⁴,Chen Jingliang⁴,Song Tianbao²
£¨1.First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China; 2.Medical School, Xi'an Jiaotong University, Xi'an 710061, China; 3.Department of Psychiatriy, Shanxi Corps Hospital, Chinese People's Armed Police, Taiyuan 030006, China; 4.School of Electrical Engineering, Xi'an Jiaotong University, Xi'an 710049, China£©

Abstract£ºThe restrained stress model of female rats in pulsed current magnetic fields (PCMFs)was established to observe the effects of PCMFs on neural stem cell quantity and proliferation in hippocampus of stressed female rats. Four groups, control, stress, PCMFs, and PCMFs+stress, were divided into, and the quantities of nestinª²positive cells and BrdUª²positive cells were contrastively counted and determined in different groups with the method of immunohistochemistry. It is found that the nestinª²positive cells are assembled in hippocampal CA1 and CA2 regions for all groups, the BrdUª²postitive cells were assembled in hippocampal dentate gyrus(DG), and the expression of nestinª² and BrdUª²positive cells in each of the other three groups is stronger than that in control group, especially in PCMFs+stress group, but no significant difference between PCMFs group and PCMFs+stress group. It is suggested that stress is capable of increasing inner neural stem cell proliferation in hippocampus of female rats, while PCMFs, as outer physical factor, enable to stimulate neural stem cell proliferation and reform the damaged neuron.
Keywords£ºpulsed current magnetic field; neural stem cell of hippocampus; stressed rat