Difference Protein Maps of Kidney Tissues of
IgA Nephropathy Rat that Arise from the Effects of Fructus Corni
Liang Heng,Xing Jianyu,Liu Xicheng,Huang Liming
(Key Laboratory of Biomedical Information Engineering of Education Ministry,Xi'an Jiaotong
University,Xi'an 710049,China)
Abstract:The distinct changes of difference protein maps of kidney tissues of IgA
nephropathy rat that arises from the effects of the traditional chinese medicine, fructus
corni, were found by two-dimensional electrophoresis (2-DE).compare the 2-DE maps between
IgA nephropathy and normal rat,664 protein spots in 1 279 samples were matched,and 263
proteins were with over two-times high difference in the matched spots,3 proteins were not
expressed and 1 protein was uniquely expressed in IgA nephropathy. In IgA nephropathy rat
fed with fructus corni extract (FCE) the 147 in the matched spots,the unexpressed 3
proteins and the uniquely expressed 1 protein were entirely recovered to normal
expressions by comparing IgA nephropathy rat without FCE.Synchronously, there is uniquely
expressed 1 protein only in the rat fed with FCE.This experiment reveals that these
difference and uniquely expressed proteins ought to be the target proteins that FCE acts
on IgA nephropathy,and FCE can effectively redress rat IgA nephropathy probably via
affecting these target proteins.
Keywords:mouse kidney tissue;two-dimensional electrophoresis;proteomics;IgA nephropathy