Cloning and Expression of DnaJ-like Gene of Bean
Zhang Yuxiu¹,Zhang Yanhong²,Yang Shuiyun¹,Chai Tuanyao²,Zhao Wenmin¹
(1. School of Life Scienee and Technology,Xi'an Jiaotong University,Xi'an 710049,China;2. Graduate School of Chinese Academy of Sciences)

Abstract: PvSR6 (Phaseolus vulgaris Stress Related)cDNA encodes a bean (Phaseolus vulgaris L.)DnaJ-like. Sequence analysis of partial genomic DNA showed no intron in its coding region. Southern blot showed that more than one PvSR6 gene copy was existed in bean genome. Northern blot revealed that PvSR6 gene was constitutively expressed in leaf tissue, however, the mRNA level was increased by heavy metals （Hg, Cd and As),excess Cu and Zn ions, and other environment stresses, such as wounded, virus infection, elevated temperature and salicylic acid . It suggested that PvSR6 might be involved in the process of plant resistance to various stresses.
Keywords: DnaJ-like protein;gene cloning;Phaseolus vulgaris